GenCheck® DNA Extraction Kit Type S/F is a kit for extracting DNA from soil and fecal samples. With extraction solution of special composition, DNA can be extracted from not only non-volcanic ash soil but also volcanic ash soil that is difficult to extract.
GenCheck® DNA Extraction Kit Type S/F combines chemical lysis method with a surfactant and a physical cell disruption method using Beads Beating to extract DNA. As a result, DNA can also be extracted from microorganisms with strong cell walls. Therefore, soil DNA and fecal DNA that reflect the actual microorganism group structure can be obtained.
DNA is collected by centrifugation using a membrane based spin column. Therefore, DNA can be extracted easily and quickly without preparing nucleic acids by ethanol precipitation.
The extracted soil DNA and fecal DNA are suitable for soil analysis and the intestinal flora analysis using next-generation sequencing and PCR-DGGE analysis to analyze microbial community structure of the microorganisms present in soil and feces.
- Apply the combination of chemical lysis method and the physical cell disruption method
- Extract DNA from microorganisms with strong cell walls using Beads Beating
- Extract DNA easily and quickly using spin column.
Example protocols
Sample Preparation
Bead tube
(up to 500mg of sample material)
(up to 500mg of sample material)
- 950μl of Lysis Solution M
- 50μl of Lysis Solution S
- Micro smash 4,600rpm, 45sec (Beads Beating)
- 1hr, 65℃
- 12,000xg, 1min, room temp.
Sample Purificatin
600μl of Supernatant
- 2μl of RNase (100 mg/mL)
- 10min, 37℃
- 400μl of Purification solution
- Mix
- 600μl of Chloroform
- Vortex 15sec
- 20,000xg, 15min, room temp
Sample Precipitation
800μl of Supernatant
- 800μl of 100% EtOH
- Mix
Sample Loading
- Load 800μl sample (contain precipitates) onto the spin column
- 13,000xg, 30sec, room temp
- Load remaining sample
- 13,000xg, 30sec, room temp
Membrane Washing
- 600μl of Wash solution1, 13,000xg, 1min, room temp
- 600μl of Wash solution1, 13,000xg, 1min, room temp
- 600μl of Wash solution2, 13,000xg, 1min, room temp
EluteDNA
Place the spin Column into a new tube
- 100μl of TE
- 3min, room temp
- 13,000xg, 1min, room temp
